Liquid crystal biosensor based on AuNPs signal amplification for detection of human chorionic gonadotropin

The detection of human chorionic gonadotropin (HCG) allows for the determination of pregnancy and is thus crucial during early pregnancy testing. This study introduces a novel liquid crystal (LC) biosensor that employs Au nanoparticles (AuNPs) for signal amplification, thus enabling the detection of the HCG antigen in a micro, efficient, and cost-effective manner. The sensor design capitalizes on the unique properties of LC to facilitate the detection of HCG. In this research, the surface of the base substrate was first modified with material from DMOAP and APTES, and EDC/sulfo-NHS was used to couple AuNPs and β-hCG to form an AuNP-β-hCG conjugate that improves the coupling rate. The carboxyl group of the antibody was reacted with the aldehyde group of glutaraldehyde, which helps to fix the β-hCG antibody to the surface of the substrate. The HCG sample is immobilized on the surface of the substrate via antigen-antibody immunobinding. As signal amplifiers, the AuNPs can have a significant effect on the topology of the interface and the vertical order of the LC molecules, thus reducing the limit of detection. Finally, the limit of detection was calculated using the SPSS system, and the relationship between grey values and concentrations was also obtained. The detection limit for HCG can be as low as 1.916 × 10^-3 mIU·mL^-1 under ideal conditions. Compared to other detection methods for HCG, this structure provides a detection pathway with excellent sensitivity, low detection limits, and better specificity, thus offering a new idea for HCG or any other target requiring trace detection.