A validated method for detection of cannflavins in hemp extracts

Area of Science:

• Analytical Chemistry
• Pharmacognosy

Background:

• Cannflavins A, B, and C are bioactive compounds found in hemp.
• Accurate quantification of these compounds is essential for research and product development.

Purpose of the Study:

• To develop and validate a selective and sensitive assay for detecting cannflavin A, B, and C.
• To apply the developed method for analyzing cannflavins in hemp extracts and topical formulations.

Main Methods:

• Liquid chromatography-mass spectrometry (LC-MS) with electrospray positive ionization.
• Use of a C-18 analytical column and a mobile phase of acetonitrile/water with formic acid.
• Quantification using a deuterated analog (cannabidiol-D3) as the internal standard.

Main Results:

• The assay demonstrated excellent linearity, sensitivity (LOD 0.25 μg/mL, LOQ 0.5 μg/mL), and selectivity.
• The total run time was less than 20 minutes.
• The method was successfully applied to analyze cannflavin release from prototype topical formulations.

Conclusions:

• A robust and sensitive LC-MS method for cannflavin analysis was established.
• The method is suitable for diverse sample matrices, including hemp extracts and topical products.
• This assay facilitates the quality control and research of hemp-derived compounds.