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Updated: Jul 19, 2025
Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model
Published on: April 2, 2021
Identification and validation of lactate metabolism-related genes in oxygen-induced retinopathy
Jiawei Xu1,2, Yunpeng Zhang1,2, Rong Gan2
1Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China.
Insights
Investigating lactate metabolism in premature infants reveals 41 key genes linked to Retinopathy of Prematurity (ROP). This research offers new insights into ROP development and potential treatments.
Area of Science:
- Ophthalmology
- Neonatology
- Biochemistry
Background:
- Retinopathy of Prematurity (ROP) is a major cause of childhood blindness in premature infants.
- Abnormal retinal vascular growth characterizes ROP, a complex multifactorial disease.
- The role of lactic acid metabolism in ROP pathogenesis is understudied.
Purpose of the Study:
- To identify genes involved in lactate metabolism related to ROP.
- To elucidate the interactions, pathways, and immune cell infiltration of these genes.
- To predict the functional mechanisms underlying ROP development.
Main Methods:
- Utilized a dataset (GSE158799) from mouse models of oxygen-induced retinopathy (OIR).
- Analyzed 284 genes to identify 41 differentially expressed lactate metabolism-related genes (LMRGs).
- Performed bioinformatics analysis to explore gene interactions, pathway enrichment, and immune cell infiltration.
Main Results:
- Identified 41 potentially differentially expressed lactate metabolism-related genes (LMRGs) associated with ROP.
- Revealed interactions, enriched pathways, and immune cell infiltration patterns among these LMRGs.
- Predicted that these genes regulate lactate metabolism, impacting metabolic and immune processes in ROP.
Conclusions:
- Lactate metabolism-related genes play a significant role in ROP development.
- Understanding these mechanisms may provide novel therapeutic targets for ROP.
- This study expands knowledge on the intrinsic mechanisms driving ROP.
Abstract:
Retinopathy of Prematurity (ROP) is a multifactorial disease characterized by abnormal retinal vascular growth in premature infants, which is one of the leading causes of childhood blindness. Lactic acid metabolism may play an imperative role in the development of ROP, but there are still few relevant studies. Our team use a dataset GSE158799 contained 284 genes in 3 P17_OIR mice and 3 P30_OIR mice to identify 41 potentially differentially expressed lactate metabolism-related genes (LMRGs) related to ROP. Then through bioinformatics analysis, we strive to reveal the interaction, the enriched pathways and the immune cell infiltration among these LMRGs, and predict their functions and internal mechanisms. These DEGs may regulate lactate metabolism, leading to the changes of metabolism and immunity, thereby inducing the development of ROP. Our results will expand our understanding of the intrinsic mechanism of ROP and may be helpful for the directions for treatment of ROP in the future.
