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Intraoperative administration of isoflurane improves survival in rats exposed to caecal ligation and puncture
Keita Ikeda1, Hari P Osuru1, Robert H Thiele1
1University of Virginia Health System, Department of Anaesthesiology, Charlottesville, VA, USA.
Background:
Emerging data suggest that volatile anaesthetic agents may be protective during critical illness.
Methods:
Three-month-old Sprague Dawley rats were randomly allocated to one of four groups: isoflurane during surgery followed by 3 days of isoflurane 0.8% (and intralipid i.v.), propofol during surgery and 314 μg kg-1 h-1 propofol for 3 days, isoflurane during surgery and intralipid for 3 days, and propofol during surgery and intralipid for 3 days. After induction with propofol or isoflurane, rats breathed oxygen 100% spontaneously via a nose cone. Propofol or intralipid was administered through a 22-gauge jugular vein i.v. catheter. Caecal ligation and puncture was performed through a paramedian incision. The surgical concentration of isoflurane was kept at 2%, and propofol was maintained at 800 μg kg-1 h-1. After recovery and 3 days of exposure to intralipid or anaesthetic agents, the rats were allowed to roam free in an adequately vented, temperature- and humidity-controlled cage with food and water ad libitum.
Results:
Rats that received isoflurane for 3 days survived longer than the postoperative propofol group (P=0.0002, log-rank test). Among rats receiving no postoperative anaesthetic, those receiving isoflurane during surgery survived longer than those that received propofol during surgery group (P=0.0081).
Conclusions:
Exposure to isoflurane, as opposed to propofol, may improve survival in rats exposed to caecal ligation and puncture.
