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Updated: Jul 19, 2025
09:03
Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation
Published on: February 7, 2012
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Glucose oxidation drives trunk neural crest cell development and fate
Nioosha Nekooie Marnany1, Redouane Fodil1, Sophie Féréol1
1Université Paris-Est Créteil, INSERM, IMRB, 94010 Créteil, France.
Journal of Cell Science
|August 17, 2023
Summary
Avian trunk neural crest cells (NCCs) require integrated glucose metabolism, including glycolysis and mitochondrial respiration, for proper embryonic development and cell fate. Glucose oxidation is crucial for their migration and differentiation.
Area of Science:
- Developmental Biology
- Cellular Metabolism
- Stem Cell Biology
Background:
- Bioenergetic metabolism critically regulates cellular functions and signaling.
- The precise role of metabolism in instructing cell behavior and fate during embryonic development is not fully understood.
- Neural crest cells (NCCs) are a migratory stem cell population vital for vertebrate embryogenesis.
Purpose of the Study:
- To investigate the role of glucose metabolism in the development of avian trunk NCCs.
- To compare the metabolic phenotypes of trunk NCCs with cranial NCCs.
- To elucidate the integrated metabolic pathways essential for trunk NCC functions.
Main Methods:
- Analysis of metabolic phenotypes in avian trunk and cranial NCCs.
- Investigation of glucose metabolism pathways, including glycolysis, mitochondrial respiration, and pentose phosphate pathway.
- Assessment of trunk NCC development in the absence of glucose and reliance on alternative pathways.
Main Results:
- Trunk NCCs exhibit prominent glucose oxidation, unlike cranial NCCs which rely on aerobic glycolysis.
- Multiple glucose metabolism pathways (glycolysis, mitochondrial respiration, pentose phosphate pathway) are integrated for trunk NCC development.
- Absence of glucose impairs trunk NCC adaptation, stemness, and fate decisions, even with pyruvate-fueled OXPHOS.
Conclusions:
- Trunk NCCs require a comprehensive utilization of glucose metabolism pathways for their development.
- Integrated glucose metabolism is essential for diverse cellular programs including migration, proliferation, and differentiation.
- Specific reliance on glucose pathways is critical for trunk NCCs to meet developmental metabolic demands.
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