Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fe<sub>3</sub>C@NC nanozyme-based platform for highly sensitive dual-readout sequential detection of cysteine and Hg<sup>2</sup>.

Mikrochimica acta·2026
Same author

GSH-activatable rare-earth nanoprobe for NIR-II fluorescence imaging-guided surgery and enhanced chemotherapy/chemodynamic therapy of breast cancer.

Cancer letters·2026
Same author

Correction: Microneedle-mediated delivery of Coptis chinensis-derived nanovesicles orchestrating antibacterial and macrophage reprogramming for comprehensive wound healing.

Journal of nanobiotechnology·2026
Same author

State-of-the-Art Applications of Field-Effect Transistor Biosensors in Exosome Detection: A Comprehensive Review.

Biosensors·2026
Same author

Electrochemical sensing of dopamine based on a Ru-CNQD/CNT/HCS nanocomposite for diagnosing Parkinson's disease.

Talanta·2026
Same author

LINC01605 Predicts the Poor Prognosis of Non-Small Cell Lung Cancer and Promotes Chemotherapy Resistance by Regulating miR-7111-5p/ELK1.

The Kaohsiung journal of medical sciences·2026

Related Experiment Video

Updated: Jul 19, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.2K

Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detection.

Kun Li1, Chunzi Liang2, Yuling Pan2

  • 1Hubei University of Chinese Medicine, Wuhan City, Hubei Province 430073, China; Department of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

STAR Protocols
|August 17, 2023
PubMed
Summary

This study details a new biosensor for detecting exosomal microRNAs (miRNAs). The ultrasensitive graphene-based sensor can distinguish cancer patients from healthy individuals by analyzing specific miRNA levels.

Keywords:
Biotechnology and BioengineeringCancerChemistry

More Related Videos

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.5K
Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement EFIRM
11:02

Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement EFIRM

Published on: January 23, 2015

18.5K

Related Experiment Videos

Last Updated: Jul 19, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

33.2K
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.5K
Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement EFIRM
11:02

Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement EFIRM

Published on: January 23, 2015

18.5K

Area of Science:

  • Biotechnology and Biomedical Engineering
  • Nanomaterials for Biosensing
  • Molecular Diagnostics

Background:

  • Exosomal microRNAs (miRNAs) are promising biomarkers for early disease detection.
  • Current detection methods often lack the sensitivity and specificity required for clinical application.
  • Development of ultrasensitive and reliable biosensors is crucial for exosomal miRNA analysis.

Purpose of the Study:

  • To present a detailed protocol for constructing an ultrasensitive biosensor for exosomal-miRNA detection.
  • To enable accurate quantification of specific exosomal miRNAs for diagnostic purposes.
  • To demonstrate the biosensor's capability in differentiating cancer patients from healthy individuals.

Main Methods:

  • Preparation of graphene quantum dot-phosphorodiamidate morpholino oligomer hybrids.
  • Deposition of hybrids onto a reduced graphene oxide field-effect surface.
  • Hybridization of target exosomal miRNA with the sensor probe, followed by electrical signal capture and analysis.

Main Results:

  • Successful construction of an ultrasensitive biosensor for exosomal-miRNA detection.
  • Optimization procedures for biosensor construction and performance evaluation were detailed.
  • The biosensor accurately quantified plasma exosomal miRNA21, enabling differentiation between cancer patients and healthy individuals.

Conclusions:

  • The developed protocol provides a robust method for creating a highly sensitive exosomal-miRNA biosensor.
  • This biosensor holds significant potential for non-invasive cancer diagnostics through plasma exosomal miRNA analysis.
  • The protocol serves as a valuable resource for researchers in the field of molecular diagnostics and biomarker discovery.