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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...
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Fluorescence detection methods for microfluidic droplet platforms
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Fluoranthene determination based on a rapid and sensitive syringe extraction and solid-phase fluorescence technique.

Jiayan Shen1, Long Li2, Kehan Xu3

  • 1College of Chemical and Molecular Engineering, East China University of Science and Technology, Shanghai, China.

Luminescence : the Journal of Biological and Chemical Luminescence
|August 17, 2023
PubMed
Summary

A new method detects fluoranthene (FL), a harmful polycyclic aromatic hydrocarbon (PAH), in water. This rapid, sensitive technique uses syringe solid-phase extraction and fluorescence spectrometry for accurate environmental monitoring.

Keywords:
FLrapid detectionsolid-phase fluorescence spectrumsyringe

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry

Background:

  • Polycyclic Aromatic Hydrocarbons (PAHs) are significant environmental pollutants.
  • Fluoranthene (FL) is a specific PAH of concern in contaminated water.
  • Accurate and rapid detection methods for FL are crucial for environmental safety.

Purpose of the Study:

  • To develop a rapid and sensitive analytical strategy for fluoranthene detection.
  • To determine FL concentrations in polycyclic aromatic hydrocarbon-polluted environmental water samples.
  • To validate a novel method combining syringe solid-phase extraction and solid-phase fluorescence spectrometry.

Main Methods:

  • Syringe solid-phase extraction (SPE) for sample enrichment.
  • Solid-phase fluorescence spectrometry for signal detection.
  • Direct fluorescence monitoring on the membrane surface post-enrichment.

Main Results:

  • Linearity observed in the 2-50 μg/L concentration range (R² = 0.998).
  • Low limit of detection achieved at 0.143 μg/L.
  • High recoveries (93.47%-109.81%) and low relative standard deviations (2.06%-6.32%) in real samples.

Conclusions:

  • The proposed method is fast, simple, portable, and highly sensitive.
  • The method demonstrates strong anti-interference capabilities.
  • This technique is suitable for rapid, on-site detection of fluoranthene in environmental monitoring.