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Morpho-cultural, pathological and molecular variability in Phloeospora maculans causing leaf spot of mulberry (Morus

Zakiya Saif1, Sabiha Ashraf2, Mehraj D Shah3

  • 1College of Temperate Sericulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu, 190025, Kashmir, India.

Molecular Biology Reports
|August 17, 2023
PubMed
Abstract

Insights

Leaf spot disease in mulberry, caused by Phloeospora maculans, shows high diversity in Kashmir. Understanding this pathogen

Area of Science:

  • Plant Pathology
  • Mycology
  • Silk Industry Research

Background:

  • Mulberry leaf spot disease (LSD), caused by Phloeospora maculans, poses a significant threat to the silk industry in Jammu and Kashmir, India.
  • Effective disease management necessitates understanding the pathogen's population structure and diversity.

Purpose of the Study:

  • To investigate the population structure and genetic diversity of Phloeospora maculans affecting mulberry in the Kashmir valley.
  • To assess variability using both conventional and molecular techniques.

Main Methods:

  • Employed conventional methods: morphological, cultural, and pathological characterization of 27 Phloeospora maculans isolates.
  • Utilized molecular methods, specifically Inter Simple Sequence Repeat (ISSR) markers, to analyze genetic variability.
  • Collected isolates from Srinagar, Bandipora, and Baramulla districts of Jammu and Kashmir.

Main Results:

  • High variability was observed among Phloeospora maculans isolates based on morpho-cultural and pathological traits.
  • ISSR markers revealed significant polymorphism, confirming their suitability for assessing genetic diversity.
  • Phylogenetic analysis clustered the 27 isolates into two distinct populations, with high genetic variation within populations (98.91%) and low variation among populations (1.08%).
  • Low genetic differentiation between populations was indicated by an Fst value of 0.01.

Conclusions:

  • A high degree of diversity exists in Phloeospora maculans at morphological, cultural, pathological, and molecular levels within the Kashmir valley.
  • The observed population structure highlights the necessity of detailed studies for successful LSD management in mulberry.
  • Findings provide crucial insights for developing targeted disease control strategies in the region.
Keywords:
ISSRLeaf spotMorus speciesPhloeospora maculansPolymerase chain reactionVariability

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