Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

The Respiratory System

The Respiratory System

The respiratory system is comprised of the organs that enable breathing. Air enters the nostrils and mouth, followed by the pharynx (throat) and larynx (voice box), which lead to the trachea (windpipe). In the thoracic cavity, the trachea splits into two bronchi that allow air to enter the lungs. The bronchi split into progressively smaller bronchioles and terminate in small groups of tiny sacs in the lungs called alveoli, where gas exchange occurs.

Regulated mRNA Transport

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Colorimetric Glucose Detection Using Copper Complexes and Filter Paper-Based Simulated Skin Analysis: Influence of Methyl Substituted Phenolato Groups.

Langmuir : the ACS journal of surfaces and colloids·2025

Same author

High-Resolution Multiplexed Sequencing of Single-Cell Full-length Transcriptome Via Combinational Barcoded Tn5 Transposon Insertion.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Same author

Enhanced RNA Preservation in Mouse Brain Tissue: A Strategy Combining Cardiac Perfusion with Hypersaline Immersion.

ACS omega·2025

Same author

snCED-seq: high-fidelity cryogenic enzymatic dissociation of nuclei for single-nucleus RNA-seq of FFPE tissues.

Nature communications·2025

Same author

Micro-region transcriptomics profiling of cerebral organoids using a capillary-based microdissection system.

Analytical methods : advancing methods and applications·2025

Same author

Microneedle Array-Based Dermal Interstitial Fluid Biopsy for Cancer Diagnosis: Advances and Challenges.

Advanced healthcare materials·2025

Same journal

A roadmap for medical large language models: a review of foundations, applications, and challenges.

Military Medical Research·2026

Same journal

The concept of neurogenic organ dysfunction is not new and should encompass all aspects of the underlying pathophysiological mechanisms.

Military Medical Research·2026

Same journal

A translational preclinical strategy for chronic spinal cord injury: neuroprotective and regenerative potential of botulinum neurotoxin type A combined with muscle atrophy prevention via electrostimulation.

Military Medical Research·2026

Same journal

Lateralized cerebral arterial blood flow and blood pressure adaptations to short-term head-down tilt: a 4D flow MRI study with cognitive function assessment.

Military Medical Research·2026

Same journal

PM2.5 as a driver of human health disorders: insights from the regulated cell death pathways.

Military Medical Research·2026

Same journal

Crosstalk in the brain tumor microenvironment: mechanisms, therapeutic strategies, and clinical advances.

Military Medical Research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 19, 2025

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

Spatial transcriptomics: recent developments and insights in respiratory research.

Wen-Jia Wang¹, Liu-Xi Chu^1,2, Li-Yong He¹

¹State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.

Military Medical Research

|August 17, 2023

Summary

This summary is machine-generated.

Spatial transcriptomics (ST) reveals respiratory system

Keywords:

Lung Spatial multi-omics Spatial transcriptomics Tumor

More Related Videos

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

Published on: May 16, 2025

Related Experiment Videos

Last Updated: Jul 19, 2025

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

Published on: May 16, 2025

Area of Science:

Respiratory Medicine
Genomics
Bioinformatics

Background:

Cellular heterogeneity in the respiratory system poses research challenges.
Bulk and single-cell RNA sequencing (scRNA-seq) offer insights but lack spatial context.
Understanding cellular interactions and localization is crucial for respiratory research.

Purpose of the Study:

To review the latest advancements in spatial transcriptomics (ST) technology.
To summarize ST applications in respiratory physiological and pathological processes, focusing on the lungs.
To propose future directions for ST in respiratory research.

Main Methods:

Review of recent iterative spatial transcriptomics technologies.
Analysis of ST applications in lung physiology and pathology.
Discussion of challenges and future prospects in ST for respiratory studies.

Main Results:

Spatial transcriptomics (ST) effectively elucidates spatial localization and cellular interactions in the respiratory system.
ST has been widely applied to various respiratory studies, enhancing understanding beyond scRNA-seq.
The review highlights ST's utility in dissecting complex lung processes.

Conclusions:

Spatial transcriptomics is a powerful tool for understanding respiratory system complexity.
Future directions include high-throughput full-length transcriptome, multi-omics integration, and advanced bioinformatics.
These advancements will drive the study of systematic mechanisms in respiratory diseases.