Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recombinant Human Decorin Normalizes the Active Features of Breast Cancer-Associated Fibroblasts.

CellsĀ·2026
Same author

E2F1 activates breast stromal fibroblasts and promotes their paracrine pro-carcinogenic effects.

Scientific reportsĀ·2025
Same author

Endogenous osteoprotegerin (OPG) represses ERĪ± and promotes stemness and chemoresistance in breast cancer cells.

Cell death discoveryĀ·2024
Same author

Decorin (DCN) Downregulation Activates Breast Stromal Fibroblasts and Promotes Their Pro-Carcinogenic Effects through the IL-6/STAT3/AUF1 Signaling.

CellsĀ·2024
Same author

Ovarian tumor cell-derived JAGGED2 promotes omental metastasis through stimulating the Notch signaling pathway in the mesothelial cells.

Cell death & diseaseĀ·2024
Same author

Ionizing radiation normalizes the features of active breast cancer stromal fibroblasts and suppresses their paracrine pro-carcinogenic effects.

Translational oncologyĀ·2023

Related Experiment Video

Updated: Jul 19, 2025

Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model
12:49

Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model

Published on: August 17, 2022

2.5K

Sheep as a Model for Liver Transplantation.

Mohammed Alsebayel1, Yasser M El-Sheikh2, Falah H Al-Mohanna3

  • 1General Surgery, Alhabib Medical Group, Riyadh, SAU.

Cureus
|August 18, 2023
PubMed
Summary

Researchers explored sheep liver transplantation feasibility. While technically challenging, this model offers an alternative when other animal models are unavailable due to cultural or religious reasons.

Keywords:
animal handlingexperimental liver transplantexperimental sheep surgeryliver preservaionporcine liver transplant

More Related Videos

Heterotopic Auxiliary Whole Liver Rat Transplant Model Utilizing a Hepaticoureterostomy for Allograft Rejection Studies
11:26

Heterotopic Auxiliary Whole Liver Rat Transplant Model Utilizing a Hepaticoureterostomy for Allograft Rejection Studies

Published on: March 8, 2024

436
Technique of Porcine Liver Procurement and Orthotopic Transplantation using an Active Porto-Caval Shunt
12:27

Technique of Porcine Liver Procurement and Orthotopic Transplantation using an Active Porto-Caval Shunt

Published on: May 7, 2015

16.5K

Related Experiment Videos

Last Updated: Jul 19, 2025

Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model
12:49

Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model

Published on: August 17, 2022

2.5K
Heterotopic Auxiliary Whole Liver Rat Transplant Model Utilizing a Hepaticoureterostomy for Allograft Rejection Studies
11:26

Heterotopic Auxiliary Whole Liver Rat Transplant Model Utilizing a Hepaticoureterostomy for Allograft Rejection Studies

Published on: March 8, 2024

436
Technique of Porcine Liver Procurement and Orthotopic Transplantation using an Active Porto-Caval Shunt
12:27

Technique of Porcine Liver Procurement and Orthotopic Transplantation using an Active Porto-Caval Shunt

Published on: May 7, 2015

16.5K

Area of Science:

  • Veterinary Surgery
  • Surgical Innovation
  • Animal Models in Research

Background:

  • Experimental liver transplantation is crucial for advancing human procedures.
  • Canine and swine models refine surgical techniques, while rodent models study metabolic and immunological aspects.
  • Sheep present a potential alternative animal model for liver transplantation research.

Purpose of the Study:

  • To evaluate the feasibility and technical aspects of performing liver transplantation in a sheep model.
  • To assess the suitability of sheep for future liver transplantation research, considering anatomical and physiological similarities to humans.

Main Methods:

  • Utilized outbred male weanling sheep (Naimi strain, 25-35 kg) as both donors and recipients under general anesthesia.
  • Performed hepatectomy in recipients, followed by portal systemic bypass construction.
  • Executed liver implantation with caval replacement, direct portal anastomosis, and aortic anastomosis of the hepatic artery.

Main Results:

  • Donor surgery was successful in all 12 cases (24 sheep).
  • Recipient surgery completion was challenging, with 3 losses during implantation due to bleeding and hemodynamic instability.
  • Five recipients died immediately post-reperfusion from bleeding; 4 survived implantation.

Conclusions:

  • Sheep liver transplantation is feasible, demonstrating potential as an animal model.
  • The model presents technical difficulties but offers an alternative for cultural/religious reasons.
  • Further technical refinement and investigation into biochemical outcomes and long-term survival are warranted.