A simple and robust LC-ESI single quadrupole MS-based method to analyze polyphenols in plant extracts using deep...

Area of Science:

• Analytical Chemistry
• Natural Product Chemistry
• Food Science

Background:

• Growing interest in health-promoting polyphenols from natural sources.
• Challenges in extracting and characterizing polyphenols due to complex plant matrices.
• Need for accessible, sensitive analytical methods for polyphenol analysis.

Purpose of the Study:

• To develop a simple, selective, and robust analytical method for a wide range of polyphenols.
• To enable polyphenol analysis using readily available single-quadrupole mass spectrometry.
• To apply the method to plant extracts obtained via green extraction.

Main Methods:

• Development of a liquid chromatography-electrospray ionization (LC-ESI) single-quadrupole mass spectrometry (MS) method.
• Analysis of 18 diverse polyphenols, including flavonoids, phenolic acids, and anthocyanins.
• Application to plant-based extracts prepared using green extraction techniques.

Main Results:

• A validated LC-ESI single-quadrupole MS method for 18 polyphenols.
• Successful application of the method to analyze polyphenols in complex plant extracts.
• Demonstration of the method's robustness and selectivity.

Conclusions:

• The developed method provides an accessible alternative to high-resolution techniques for polyphenol quantification.
• This approach facilitates the characterization of polyphenols in plant-based materials.
• It supports research into the health benefits of natural products.