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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Jul 19, 2025

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Published on: November 8, 2017

A precise design strategy for a cell-derived extracellular matrix based on CRISPR/Cas9 for regulating neural stem

Yuanxin Zhai¹, Lingyan Yang¹, Wenlong Zheng²

¹CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics. Chinese Academy of Sciences, Suzhou, Jiangsu 215123, China. lyy2013@sinano.ac.cn.

Biomaterials Science

|August 18, 2023

Summary

Researchers engineered a cell-derived extracellular matrix (ECM) that enhances growth factor binding. This functional ECM supports neural stem cell (NSC) maintenance, proliferation, and differentiation, offering biomaterial applications.

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Last Updated: Jul 19, 2025

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

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Published on: November 8, 2017

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Published on: June 2, 2020

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Published on: May 28, 2015

Area of Science:

Biomaterials Science
Stem Cell Biology
Extracellular Matrix Engineering

Background:

The extracellular matrix (ECM) is crucial for stem cell functions, including survival, proliferation, and differentiation.
Heparan sulfate proteoglycans (HSPGs), a component of the ECM, bind growth factors and morphogens, influencing development and physiology.
Developing functional ECMs is vital for regenerative medicine and research applications.

Purpose of the Study:

To engineer a cell-derived ECM functionalized with Heparan Sulfate Proteoglycan 2 (HSPG2).
To evaluate the capacity of the engineered ECM to bind basic fibroblast growth factor (bFGF).
To assess the impact of the functionalized ECM on neural stem cell (NSC) behavior.

Main Methods:

Overexpression of endogenous HSPG2 in mouse embryonic fibroblasts using CRISPR/Cas9 synergistic activation mediator system.
Fabrication of a cell-derived HSPG2 functional ECM (ECM*).
Assessment of bFGF binding affinity to ECM* compared to control ECM.
Culturing neural stem cells (NSCs) on ECM* with varying bFGF concentrations.

Main Results:

The engineered ECM* demonstrated enhanced enrichment of bFGF compared to control ECM.
ECM* exhibited stronger binding affinity for bFGF.
Increased bFGF concentrations on ECM* significantly improved NSC stemness maintenance, proliferation, and differentiation.

Conclusions:

Engineered cell-derived ECM functionalized with HSPG2 can effectively enrich and bind bFGF.
This functional ECM supports and enhances key neural stem cell behaviors in vitro.
The study presents a precise strategy for designing cell-derived ECM biomaterials for research and regenerative medicine.