Unique Material Properties of Bombyx mori Silk Fiber Incorporated with 3-Azidotyrosine

Silk fiber produced by the silkworm Bombyx mori is a nature-derived proteinous fiber with excellent mechanical strength and broad biocompatibility. To alter its material properties and make it more suitable for textile, biomedical, and electronics applications, chemical modifications and genetic engineering methods have been extensively studied. Here, we report that the translational incorporation of a synthetic amino acid, 3-azidotyrosine (3-AzTyr), into B. mori silk fiber can improve its material properties. Such an incorporation considerably increased the fiber's mechanical strength and remarkably changed its solubility, whereas its crystalline hierarchical structure was not perturbed, as shown by X-ray analyses. These changes were probably caused by the intra- and/or intermolecular crosslinkings involving the azido group of 3-AzTyr during the degumming process to remove a coating protein. These findings indicate that the incorporation of synthetic amino acids could be an efficient method to improve the properties of silk-based materials.