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Related Experiment Video

Updated: Jul 18, 2025

Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care

Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care

Published on: February 16, 2015

Challenges and new technologies in adoptive cell therapy.

Pengchao Zhang^1,2, Guizhong Zhang³, Xiaochun Wan⁴

¹Center for Protein and Cell-based Drugs, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Avenue, Nanshan District, Shenzhen, 518055, People's Republic of China.

Journal of Hematology & Oncology

|August 18, 2023

Summary

Chimeric antigen receptor (CAR)-T cell therapy shows promise for blood cancers but has limitations. New CAR-engineered immune cells like CAR-NK and CAR-macrophages offer potential solutions for improved cancer immunotherapy.

Keywords:

Adoptive cell therapy Cancer therapy Chimeric antigen receptor Gene transduction T cell receptor

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Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care

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Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

Published on: December 23, 2022

Area of Science:

Immunology
Oncology
Biotechnology

Background:

Adoptive cell therapies (ACTs) have evolved significantly, with chimeric antigen receptor (CAR)-T cell therapy revolutionizing hematologic malignancy treatment.
Despite successes, CAR-T cell therapy faces challenges in practicality and toxicity in both autologous and allogeneic applications.

Purpose of the Study:

To review the development, advantages, and challenges of novel CAR-engineered immune cell therapies beyond CAR-T cells.
To discuss gene transduction strategies in immunotherapy and explore future directions for cancer treatment.

Main Methods:

Review of existing literature on adoptive cell therapies, focusing on CAR engineering.
Analysis of emerging CAR-based cell therapies including CAR-NK, CAR-macrophage, CAR-γδT, and CAR-NKT cells.
Discussion of gene transduction techniques and strategies for creating positive feedback immune circuits.

Main Results:

CAR engineering has been extended to various immune cells, leading to CAR-NK, CAR-macrophage, CAR-γδT, and CAR-NKT therapies.
Gene transduction strategies are crucial for effective immune cell engineering in immunotherapy.
Positive feedback immune circuits may overcome limitations of single-cell type ACTs.

Conclusions:

Novel CAR-engineered immune cells offer potential to overcome CAR-T cell therapy limitations.
Advanced gene transduction and the development of immune circuits are key for future cancer immunotherapy.
Expanding CAR technology to diverse immune cells holds significant therapeutic promise.