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A project-oriented biochemistry laboratory for protein engineering and structure-function using small laccase enzyme
Arie Van Wieren1, Philip Colen1, Sudipta Majumdar1
1Madia Department of Chemistry, Biochemistry, Physics and Engineering, Indiana University of Pennsylvania, Indiana, Pennsylvania, USA.
Summary
This study developed a project-based biochemistry lab course to teach protein structure-function relationships. Students gained practical skills and enthusiasm for biochemical research through hands-on protein engineering.
Area of Science:
- Biochemistry
- Molecular Biology
- Biotechnology
Background:
- Understanding protein structure-function is crucial for biochemical research.
- Integrating bioinformatics and protein engineering tools enhances this understanding.
- Prior knowledge of recombinant protein technology can be leveraged for advanced studies.
Purpose of the Study:
- To develop a semester-long, project-oriented laboratory experience for teaching protein structure-function relationships.
- To integrate bioinformatics and protein engineering concepts into a cohesive learning module.
- To enhance student learning and enthusiasm in biochemistry.
Main Methods:
- A four-module course structure: protein structure visualization/modification, mutagenesis target identification, site-directed mutagenesis, and mutant protein expression, purification, and characterization.
- Utilized the small laccase (SLAC) protein, previously cloned by students.
- Employed a goal-oriented, project-based learning approach in a research-like laboratory setting.
Main Results:
- Students successfully applied prior knowledge of recombinant protein technology to new techniques.
- The project-based approach facilitated understanding of protein structure-function relationships.
- Pre- and post-course assessments indicated increased student learning and enthusiasm.
Conclusions:
- The developed laboratory course effectively teaches protein structure-function relationships.
- Project-based learning enhances student engagement and skill acquisition in biochemistry.
- This approach provides a valuable model for biochemistry laboratory education.
