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Updated: Jul 18, 2025

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
Published on: April 20, 2017
Using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset
Angela R Bunning1, Samuel J Anderson1, Mohan L Gupta1
1Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA 50010, USA.
Abstract:
The microtubule (MT)-stabilizing drug Taxol (paclitaxel) is a commonly used tool to investigate MT dynamics and MT-dependent processes. Here, we present a protocol for using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset. We describe steps for establishing a log phase culture, synchronizing cells in G1, arresting in metaphase, and releasing cells into Taxol. We then detail procedures for imaging and scoring anaphase onset. This protocol facilitates maintenance and reproducibility in testing drug-sensitized and Taxol-sensitized yeast strains. For complete details on the use and execution of this protocol, please refer to Proudfoot et al.1.
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Anaphase A and B
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...

