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Updated: Jul 18, 2025

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Published on: May 27, 2014
An Introduction to Analytical Challenges, Approaches, and Applications in Mass Spectrometry-Based Secretomics
Sascha Knecht1, H Christian Eberl2, Norbert Kreisz3
1Omics Sciences, Genomic Sciences, GlaxoSmithKline, Heidelberg, Germany; Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
Abstract:
The active release of proteins into the extracellular space and the proteolytic cleavage of cell surface proteins are key processes that coordinate and fine-tune a multitude of physiological functions. The entirety of proteins that fulfill these extracellular tasks are referred to as the secretome and are of special interest for the investigation of biomarkers of disease states and physiological processes related to cell-cell communication. LC-MS-based proteomics approaches are a valuable tool for the comprehensive and unbiased characterization of this important subproteome. This review discusses procedures, opportunities, and limitations of mass spectrometry-based secretomics to better understand and navigate the complex analytical landscape for studying protein secretion in biomedical science.
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