Weak magnetic field enhances waste molasses-driven denitrification during wastewater treatment

Waste molasses, the abundant byproducts of the sugar industry, is a cost-efficient carbon source for advanced denitrification. However, the efficiency of waste molasses-driven denitrification is limited by its complex carbon content, hindering its practical application. Weak magnetic field (WMF) is reported to enhance biological nitrogen removal, but its effects on molasses-driven denitrification remains unknown. This study investigated whether the WMF can enhance waste molasses-driven nitrogen removal and explore the underlying mechanisms. It was found that WMF significantly facilitated waste molasses-driven denitrification, with total nitrogen removal efficiency increased by 1.25 times (from 77% to 96%). WMF stimulated the nitrate reductase's activity by 7-18%, and the enhancement was improved as WMF intensified. Quantitative qPCR analysis indicated that the abundances of denitrifying enzymes increased under WMF, which was consistent with the proliferation of denitrifying bacteria Denitratisoma and Devosia. This study has demonstrated that WMF is promising for enhancing complex carbon-driven denitrification processes.