Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Omics Approaches to Unveil Biotic Stress Responses in Olive: Current Knowledge and Future.

Journal of experimental botany·2026

Same author

Exploring Metabolic Interaction between Ophiostoma novo-ulmi and Geosmithia spp.

Microbial ecology·2026

Same author

A viroid-like RNA can be transmitted among different <i>Trichoderma</i> species affecting their antagonistic capacity.

mBio·2026

Same author

Olive orchard intensification compromises soil water erosion control in a semi-arid environment.

PloS one·2026

Same author

Development of a Rapid and Sensitive RT-qPCR for the Specific Detection of Citrus Viroid V and Its Field Application.

Viruses·2026

Same author

Evaluation of antifungal and antimycotoxigenic activity of selected natural antioxidants against Fusarium culmorum: in vitro and in planta assays.

Journal of the science of food and agriculture·2026

Search research articles

Related Experiment Video

Updated: Jul 18, 2025

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

Next-generation methods for early disease detection in crops.

Daniela Trippa¹, Riccardo Scalenghe¹, Marcos Fernando Basso²

¹Dipartimento di Scienze Agrarie Alimentari e Forestali, Università degli Studi di Palermo, Palermo, Italy.

Pest Management Science

|August 20, 2023

Summary

Early detection of plant pathogens is crucial for effective disease management. This review compares traditional methods like ELISA and nucleic acid-based techniques with innovative field-deployable tools such as lateral flow systems and portable PCR.

Keywords:

LAMP PCR diagnosis early detection in-field portable devices plant disease plant pathogens

More Related Videos

A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant–Environment Interactions

A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant–Environment Interactions

Published on: August 5, 2020

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

Published on: January 3, 2014

Related Experiment Videos

Last Updated: Jul 18, 2025

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant–Environment Interactions

A Telemetric, Gravimetric Platform for Real-Time Physiological Phenotyping of Plant–Environment Interactions

Published on: August 5, 2020

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

Published on: January 3, 2014

Area of Science:

Plant Pathology
Agricultural Science
Biotechnology

Background:

Accurate identification of plant pathogens is vital for disease control and preventing agricultural losses.
Traditional diagnostic methods often lack sensitivity, especially during early or asymptomatic infection stages.
Efficient pathogen detection underpins effective management strategies to mitigate disease spread and impact.

Purpose of the Study:

To review and discuss traditional and innovative methods for early plant pathogen detection.
To highlight the advantages and limitations of various diagnostic techniques.
To provide insights into current methodologies for effective plant pathogen identification.

Main Methods:

Review of traditional diagnostic techniques including serological methods (e.g., ELISA) and molecular methods (DNA, RNA).
Evaluation of innovative detection tools such as lateral flow systems and portable PCR assays.
Discussion of pathogen detection based on pathogen components like nucleic acids and proteins.

Main Results:

Enzyme-linked immunosorbent assay (ELISA) offers high-throughput and low cost but lacks sensitivity for asymptomatic infections.
Nucleic acid-based technologies are reliable for non-cultivable pathogens.
Innovative tools like lateral flow systems and portable PCR provide rapid, in-field results but require further sensitivity improvements.

Conclusions:

Portable diagnostic instruments have the potential to accelerate pathogen detection, reduce costs, and transform plant pathology practices.
A comprehensive understanding of method advantages and limitations is key to selecting appropriate detection strategies.
Continued innovation in diagnostic tools is essential for timely and accurate plant pathogen identification.