Related Experiment Video
Updated: Aug 10, 2026
Non-invasive Assessment of Microvascular and Endothelial Function
Published on: January 29, 2013
Sublingual microcirculation in healthy pediatric population using the sidestream dark-field imaging method
Vlasta Krausova1,2, David Neumann2,3,4, Jaroslav Kraus5
1Department of Pediatrics, Masaryk Hospital, Krajska Zdravotni, Usti nad Labem, Czech Republic.
Background:
The sidestream dark-field imaging method is used to study microcirculation. Normal values of sublingual microcirculation parameters in healthy children of different age and gender categories are unknown.
Objective:
The study's main goal was to determine normal values of selected parameters of sublingual microcirculation in healthy children of different age and gender categories.
Methods:
40 healthy children were measured, ten aged 3-5.9 years, ten aged 6-10.9 years, ten aged 11-14.9 years, and ten aged 15-18.9 years. After recording the basic anthropometric parameters and vital functions, each volunteer had their microcirculation measured using an SDF probe placed sublingually. Three video clips were recorded and processed offline, and the three best and most stable parts of each were analyzed.
Results:
Total vascular density, small vessel density, proportion of perfused small vessels, perfused vessel density, perfused small vessel density, and DeBacker's score were significantly higher in females than in males. There were no differences between age groups in microcirculation parameters except MFI.
Conclusions:
Age does not influence normal values of microcirculatory parameters. Female gender was associated with higher vessel density, perfused vessel density, and DeBacker's score. A suggestion of the normal range of microcirculatory parameters in healthy children is provided.
