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Rapid Visual LAMP Method for Detection of Genetically Modified Organisms
Yujun Xing1, Jie Liang2, Fei Dong2
1Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology/Key Laboratory for Control/Technology and Standard for Agro-Product Safety and Quality, Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety/Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
A new DNA extraction and detection method using polyacrylamide microparticles (PAMMPs) enables rapid, sensitive, and cost-effective identification of genetically modified organisms (GMOs) in food.
Area of Science:
- Biotechnology
- Molecular Biology
- Food Science
Background:
- Genetically modified organisms (GMOs) require reliable detection methods.
- Existing methods for GMO detection can be time-consuming and costly.
Purpose of the Study:
- To develop a novel, rapid, and cost-effective method for GMO detection.
- To utilize polyacrylamide microparticles (PAMMPs) for DNA capture and amplification.
Main Methods:
- Developed a loop-mediated isothermal amplification (LAMP) method using DNA captured on PAMMPs (PAMMPs@DNA-LAMP).
- Designed specific LAMP primers targeting the cauliflower mosaic virus 35S (CaMV35S) promoter.
- Validated the method's performance using conventional PCR, quantitative PCR (qPCR), and real-world food samples.
Main Results:
- Achieved rapid DNA extraction (5-10 min) and amplification (approx. 30 min) at a constant 63 °C.
- Demonstrated high specificity and sensitivity, detecting down to 0.01% target sequences.
- Successfully applied the PAMMPs@DNA-LAMP method to detect GMOs in major crop food samples (soybean, maize, rice).
Conclusions:
- The PAMMPs@DNA-LAMP assay offers a sensitive, specific, and rapid alternative to conventional PCR for GMO detection.
- This method provides a novel approach for field-based GMO analysis.
- The technique is efficient, cost-effective, and suitable for practical food sample analysis.
