Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanical loading primes MSC-derived exosomes to promote cartilage repair.

Bioactive materials·2026
Same author

Gut microbiota and its metabolites with thyroid diseases: functions and mechanisms.

Frontiers in immunology·2026
Same author

Predicting STAS in peripheral stage I lung adenocarcinoma: the incremental value of CT-based tumor disappearance rate, with a focus on part-solid nodules.

BMC medical imaging·2026
Same author

Standardized debridement for the management of fracture-related infection after intramedullary nailing: A retrospective single-center cohort study of 69 patients.

Medicine·2026
Same author

Electroacupuncture prevents CUMS induced depressive-like behaviors by inhibiting microglia-mediated synaptic pruning induced by gut dysbiosis.

Chinese medicine·2026
Same author

High-throughput screening of two-dimensional ferromagnetic materials with high Curie temperatures.

Nanoscale·2026

Related Experiment Video

Updated: Jul 18, 2025

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
09:36

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

4.9K

A portable system for economical nucleic acid amplification testing.

Hui Dong1,2, Jin Mo1,2, Yongjian Yu1,2

  • 1School of Mechanical Engineering and Automation, Fuzhou University, Fuzhou, China.

Frontiers in Bioengineering and Biotechnology
|August 21, 2023
PubMed
Summary

A new digital point-of-care testing system using microfluidic paper-based analytical devices (μPADs) can rapidly detect SARS-CoV-2 gene fragments. This cost-effective COVID-19 diagnostic tool offers high sensitivity and low reagent use for pandemic control.

Keywords:
NAATPOCTSARS-CoV-2 template detectionautomatic image processingpaper microfluidics

More Related Videos

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.3K
Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

1.9K

Related Experiment Videos

Last Updated: Jul 18, 2025

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
09:36

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

4.9K
Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.3K
Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

1.9K

Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Point-of-Care Testing

Background:

  • Rapid and large-scale pathogen screening is essential for pandemic control, as highlighted by the COVID-19 pandemic.
  • Existing methods for detecting SARS-CoV-2 gene fragments can be resource-intensive.
  • Development of efficient and accessible diagnostic tools is crucial for global health security.

Purpose of the Study:

  • To develop a digital point-of-care testing (POCT) system for the rapid detection of SARS-CoV-2 gene fragments.
  • To utilize microfluidic paper-based analytical devices (μPADs) integrated with temperature tuning and fluorescent detection.
  • To create an intelligent system with autonomous image acquisition and self-recognition for efficient analysis.

Main Methods:

  • The study employed a nucleic acid amplification test (NAAT) based POCT system.
  • Microfluidic paper-based analytical devices (μPADs) were used for detecting synthesized SARS-CoV-2 gene fragments (ORF1ab, N, and E genes).
  • The system was optimized for minimal reagent consumption (2.2 μL) and low power dissipation (6.4 W).

Main Results:

  • The developed POCT system achieved a limit of detection of 1000 copies/mL, equivalent to detecting a single RNA template per reaction.
  • Accurate quantification of target templates was possible using standard curve analysis.
  • The system demonstrated significantly lower reagent consumption compared to conventional in-tube methods.

Conclusions:

  • The digital POCT system offers a promising solution for rapid, reliable, and cost-effective detection of SARS-CoV-2 gene fragments.
  • Its low reagent consumption and compatibility with other diagnostic techniques make it suitable for resource-limited settings.
  • This technology can enhance pandemic preparedness and response capabilities, particularly in underserved areas.