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Outlook for CRISPR-based tuberculosis assays now in their infancy
Zhen Huang1, Guoliang Zhang1, Christopher J Lyon2,3
1National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, Shenzhen, Guangdong, China.
Frontiers in Immunology
|August 21, 2023
Summary
Tuberculosis (TB) diagnosis faces challenges despite molecular advancements. This review critically assesses CRISPR-based TB assays, proposing improvements for better clinical evaluation and accessibility.
Area of Science:
- Microbiology
- Molecular Diagnostics
- Public Health
Background:
- Tuberculosis (TB) is a significant global health issue, causing millions of cases and deaths annually.
- Rapid molecular diagnostic tests have improved TB detection, yet traditional methods still present challenges.
- A critical review of diagnostic approaches, particularly CRISPR-based assays, is needed.
Conclusions:
- Improvements in sample pretreatment, method development, and clinical validation are crucial for CRISPR-based TB assays.
- Standardized validation protocols and enhanced accessibility are recommended to streamline future studies.
- Addressing these aspects will facilitate the effective translation of CRISPR technology into clinical practice for TB diagnosis.
