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Photoactivatable Fluorophores for Bioimaging Applications.

Yang Zhang1, Yeting Zheng2, Andrea Tomassini2

  • 1Program of Polymer and Color Chemistry, Department of Textile Engineering, Chemistry and Science, North Carolina State University, Raleigh, North Carolina 27606, United States.

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|August 21, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed new photoactivatable fluorophores that switch between emissive states. This allows real-time imaging of cellular processes and structures with nanometer resolution, advancing biological and medical research.

Keywords:
fluorescence photoactivation and dissipation (FPD)photoactivatable fluorophores (PAFs)photoactivated localization microscopy (PALM)photochemical barcoding, single-molecule localization microscopy (SMLM)single-particle tracking photoactivated localization microscopy (spt-PALM)

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Area of Science:

  • Molecular Biology
  • Biophysics
  • Cellular Imaging

Background:

  • Photoactivatable fluorophores enable spatiotemporal control of fluorescence for imaging dynamic cellular events.
  • Current fluorophores activate from a non-emissive to an emissive state, preventing pre-activation imaging.
  • Nanometer resolution imaging is crucial for understanding cellular processes in biology and medicine.

Purpose of the Study:

  • To develop novel photoactivatable fluorophores with spectrally resolved fluorescence interconversion.
  • To enable pre-activation visualization and real-time monitoring of molecular dynamics.
  • To advance high-resolution imaging capabilities for cellular and molecular investigations.

Main Methods:

  • Synthesis of a new family of photoactivatable fluorophores.
  • Demonstration of spectrally resolved fluorescence interconversion.
  • Application in live biological systems, including developing embryos and nematodes.

Main Results:

  • Developed photoactivatable fluorophores that interconvert between emissive states.
  • Enabled real-time monitoring of molecular diffusion in developing embryos.
  • Facilitated tracking of molecules in live nematodes and visualization of cellular organelles.

Conclusions:

  • The new fluorophores offer enhanced imaging capabilities beyond traditional photoactivation.
  • These tools allow for unprecedented real-time observation of molecular and cellular dynamics.
  • The developed fluorophores are valuable for investigating molecular mechanisms regulating cell function and structure.