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Updated: Jul 18, 2025
Purification of High Yield Extracellular Vesicle Preparations Away from Virus
Published on: September 12, 2019
Methods of Isolation and Purification of Extracellular Vesicles from Different Biological Matrixes: Special Issue at
1Department of Endocrinology and Nutrition August Pi i Sunyer Biomedical Research Institute - IDIBAPS, Hospital Clínic of Barcelona; Biomedical Network Research Centre on Obesity and Nutrition Physiopathology (CIBEROBN), Instituto de Salud Carlos III; gchiva@recerca.clinic.cat.
Articles Discussed:
Hanine El Itawi, H., et al. Isolation of Splenic Microvesicles in a Murine Model of Intraperitoneal Bacterial Infection. J Vis Exp. 186, DOI: 10.3791/63480 (2022) Jones, M.T., Manioci S.W., S1, Russell, A.E. Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media. J Vis Exp. 186, DOI: 10.3791/63614 (2022) Ryan, J.M., Dobos, K.M., Kruh-Garcia, N.A. Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography. J Vis Exp. 186, DOI: 10.3791/63895 (2022) Valle-Tamayo, N., et al. Enrichment of Astrocyte-Derived Extracellular Vesicles from Human Plasma J Vis Exp. 186, DOI: 10.3791/64107 (2022) Koh, B., et al. A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells J Vis Exp. 186, DOI: 10.3791/64106 (2022).
Insights
Researchers explored methods for isolating extracellular vesicles (EVs) from various biological samples, including bacterial infection models and human plasma. These techniques are crucial for understanding EV function in health and disease.
Area of Science:
- Biotechnology
- Cell Biology
- Immunology
Background:
- Extracellular vesicles (EVs) are crucial mediators of intercellular communication.
- Efficient isolation of EVs is essential for studying their biological roles.
- Current methods for EV isolation face challenges in purity and yield.
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