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Related Concept Videos

Hypodermis01:02

Hypodermis

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The hypodermis (the subcutaneous layer or superficial fascia) is present directly below the dermis. It connects the skin to the underlying fascia (fibrous tissue) of the bones and muscles. It is not strictly a part of the skin, although the border between the hypodermis and dermis can be difficult to distinguish. The hypodermis consists of well-vascularized, loose, areolar connective tissue and adipose tissue, which functions as a mode of fat storage and provides insulation and cushioning for...
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Functions of Connective Tissues01:17

Functions of Connective Tissues

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Connective tissues perform a broad range of functions in the body. Their primary function is to connect and link different tissues in the body and act as packaging material between tissues. The areolar tissue, a connective tissue prototype, commonly cements various tissue types in diverse body organs. In contrast, adipose tissue cushions internal organs while insulating the body from heat loss.
Hard connective tissues, such as bones and cartilage, provide structure and support to the body.
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Loose Connective Tissue01:26

Loose Connective Tissue

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Loose connective tissue is found between many organs. Its main function is to absorb shock and bind tissues together. It also allows water, salts, and various nutrients to diffuse into cells that are embedded in it or present in adjacent tissues.
Adipose Tissue
Adipose tissue consists primarily of fat storage cells called adipocytes and little extracellular matrix. A large number of capillaries present within adipose tissue allow rapid mobilization of lipid molecules. White adipose tissue is...
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Overview of Lipid Metabolism01:24

Overview of Lipid Metabolism

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Lipid metabolism is a crucial process in the human body that involves the synthesis and degradation of lipids. This process is essential for energy production, cell membrane formation, and hormone production, among other functions.
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...
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Liver Physiology01:30

Liver Physiology

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The liver, an essential organ in the human body, performs over 200 vital functions that can be broadly categorized into metabolic, hematological, endocrine regulation, and bile production.
Metabolic Regulation:
The liver is the central organ involved in regulating blood composition. It stabilizes blood glucose levels, maintaining them within the range of  70–110 mg/dL. When these levels drop, the liver breaks down glycogen reserves and releases glucose into the bloodstream. It can...
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Overview of Fatty Acid Metabolism01:28

Overview of Fatty Acid Metabolism

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Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
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Related Experiment Video

Updated: Jul 18, 2025

Identification and Dissection of Diverse Mouse Adipose Depots
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Exploring Adipose Tissue Functions.

Jennifer Jager1, Jérôme Gilleron2

  • 1Team Cellular and Molecular Pathophysiology of Obesity and Diabetes, C3M, Université Côte d'Azur, Inserm.

Journal of Visualized Experiments : Jove
|August 21, 2023
PubMed
Summary

This collection of studies details methods for isolating and characterizing various adipose tissue cells and structures. Researchers can now better investigate adipose tissue biology, cell function, and disease-related remodeling.

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Related Experiment Videos

Last Updated: Jul 18, 2025

Identification and Dissection of Diverse Mouse Adipose Depots
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Area of Science:

  • Adipose tissue biology and cell isolation techniques.
  • Stromal vascular fraction and adipocyte characterization.
  • 3D imaging and network analysis in adipose tissue research.

Background:

  • Adipose tissue is crucial for energy homeostasis and endocrine function.
  • Understanding adipose tissue cellular composition and structure is vital for metabolic research.
  • Current methods enable detailed analysis of adipocytes, progenitor cells, and stromal subpopulations.

Discussion:

  • Various protocols are presented for isolating specific cell types from adipose tissue, including progenitor cells, adipocytes, and stromal vascular fractions.
  • Techniques cover both murine and human visceral adipose tissue, facilitating comparative studies.
  • Advanced imaging and cell culture models are highlighted for exploring adipose tissue structure and function.

Key Insights:

  • Established protocols allow for the isolation of viable adipocytes and stromal vascular fractions suitable for molecular analysis.
  • Methods for preparing adipose progenitor cells and characterizing stromal cell subpopulations are detailed.
  • 3D imaging and cell culture models offer new avenues for studying adipose tissue remodeling and function.

Outlook:

  • These methods provide a foundation for further research into obesity, cachexia, and other adipose tissue-related pathologies.
  • Future studies can leverage these techniques to explore the role of extracellular vesicles and cellular networks in adipose tissue.
  • Advancements in adipose tissue research will contribute to a deeper understanding of metabolic diseases and potential therapeutic targets.