Related Experiment Video

Updated: Jul 18, 2025

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
08:32

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Published on: February 12, 2019

13.8K

Extracellular Vesicles and Cardiac Aging

Fernando A C Seara1,2, Leonardo Maciel1,3, Tais Hanae Kasai-Brunswick1,4

  • 1Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

Advances in Experimental Medicine and Biology
|August 21, 2023
PubMed

Insights

Aging populations face health challenges, especially cardiovascular disease. Senescent cells release extracellular vesicles (EVs) that contribute to heart aging, but stem cell EVs may offer anti-aging therapies.

Area of Science:

  • Cardiovascular Science
  • Gerontology
  • Cell Biology

Background:

  • Global population aging presents significant health and socioeconomic challenges.
  • Cardiovascular disease is the leading cause of mortality in the elderly.
  • Accumulation of cardiac senescent cells and their secreted factors contribute to age-related cardiovascular decline.

Purpose of the Study:

  • To investigate the role of senescent cells and their secreted extracellular vesicles (EVs) in cardiovascular aging.
  • To explore the potential of EVs as biomarkers for aging-related conditions.
  • To examine the therapeutic potential of stem and progenitor cell-derived EVs for anti-aging strategies.

Main Methods:

  • Analysis of senescence-associated secretory phenotype (SASP) compounds released by senescent cells.
  • Characterization of extracellular vesicles (EVs) secreted by senescent cells.
  • Investigation of EV uptake by recipient cells and their effects on cellular function and extracellular matrix.
  • Exploration of EVs from stem and progenitor cells for potential rejuvenating effects.

Main Results:

  • Senescent cells release EVs containing SASP factors and damaged molecules.
  • These EVs can be internalized by neighboring cells, causing damage and promoting fibrosis and vascular calcification.
  • The molecular signature of aging-related EVs is variable, suggesting biomarker potential.
  • EVs from stem and progenitor cells may possess rejuvenating properties.

Conclusions:

  • Senescent cell-derived EVs play a critical role in age-related cardiovascular dysfunction.
  • EVs represent a potential source for novel aging biomarkers.
  • Stem and progenitor cell-derived EVs hold promise for future anti-aging therapies.

Keywords:
AgingCardiac agingExtracellular vesiclesSenescence

More Related Videos

Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice08:13

Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice

Published on: April 10, 2019

6.1K
Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart11:53

Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart

Published on: June 14, 2020

7.5K

Related Concept Videos

Overview of Exosomes01:36

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K
Heart Failure II: Pathophysiology01:29

Heart Failure II: Pathophysiology

Systolic Heart Failure and Compensatory MechanismsSystolic heart failure (also termed HFrEF, Heart Failure with Reduced Ejection Fraction) is the most prevalent type of heart filure. It results in a decreased volume of blood being pumped from the ventricle. The aortic arch and carotid sinuses have baroreceptors that detect reduced blood pressure, triggering the sympathetic nervous system (SNS) to release epinephrine and norepinephrine. Initially, this response aims to boost heart rate and...
15
Pathophysiology of Heart Failure01:17

Pathophysiology of Heart Failure

Heart failure (HF) is a progressive syndrome involving ventricles that leads to inadequate cardiac output. It can be classified based on location and output or ejection fraction. Ejection fraction (EF) is an essential measurement in the diagnosis and surveillance of HF. Reduced EF corresponds to systolic heart failure (HFrEF). However, HF with preserved ejection fraction (HFpEF) is becoming increasingly prevalent. Also known as diastolic HF, this form of HF is related to aging. The...
1.6K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.6K