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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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In-vitro Mutagenesis01:16

In-vitro Mutagenesis

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Updated: Jul 18, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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A Primer on Gene Editing.

Allison Cushman-Vokoun1, Ryan J Schmidt2,3, Matthew Charles Hiemenz4

  • 1From the Department of Pathology & Microbiology, University of Nebraska Medical Center, Omaha (Cushman-Vokoun).

Archives of Pathology & Laboratory Medicine
|August 21, 2023
PubMed
Summary
This summary is machine-generated.

Gene editing therapies offer transformative potential in medicine but require pathologist involvement. This educational paper aims to equip pathologists with knowledge on gene editing technologies, risks, and their role in diagnostics and therapy monitoring.

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Area of Science:

  • * Exploration of gene editing technologies and their applications in medicine.
  • * Focus on the intersection of advanced biotechnology and clinical practice.

Background:

  • * Gene editing therapies are emerging for oncology, inherited diseases, and infectious diseases.
  • * These advancements stem from natural principles but raise concerns regarding risks, cost, and ethics.

Purpose of the Study:

  • * To educate pathologists on gene editing technologies, indications, and risks.
  • * To inform about regulatory and practical issues impacting laboratory medicine.
  • * To advocate for pathologist engagement in gene editing discussions.

Main Methods:

  • * Convening of a Gene Editing Workgroup comprising pathologists.
  • * Facilitation by the College of American Pathologists Personalized Health Care Committee.
  • * Literature review and group discussions to identify knowledge gaps.

Main Results:

  • * Identified gaps in pathologists' understanding of gene editing.
  • * Summarized key topics impacting pathology and laboratory medicine.
  • * Highlighted the need for pathologists as stakeholders in gene editing implementation.

Conclusions:

  • * Gene editing represents a complex and transformative medical field.
  • * This article serves as an introduction for pathologists.
  • * Aims to facilitate informed discussions and potential practice adjustments in pathology.