Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

380
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...
380
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.5K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.5K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

1.1K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
1.1K
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

915
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
915
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

799
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
799
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

822
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
822

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic neutrophil lipidome remodeling during induction of NETosis.

Science advances·2026
Same author

Single-Ion Imaging Native Mass Spectrometry: Unraveling the Structural Features and Dissociation Energetics of Macromolecular Assemblies.

Journal of the American Society for Mass Spectrometry·2026
Same author

Microencapsulated docosahexaenoic acid increases the Omega-3 Index and attenuates the physiological impact of eccentric exercise in physically trained adults: a 12-week double-blind placebo-controlled trial.

European journal of nutrition·2026
Same author

Metabolomics across scales: from single cells to population studies.

Nature·2026
Same author

Exploring Fluoroquinolone Protomer Populations Formed with MALDI and Plasma Post-Ionization Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same author

Systematic analyses of lipid mobilization by human lipid transfer proteins.

Nature·2026
Same journal

Spatial metabolomics in medicinal plant research: current advances, technical challenges, and future perspectives.

Analytical methods : advancing methods and applications·2026
Same journal

Simultaneous determination of 22 carbamate pesticides and their metabolites in biological samples using ultrasound-assisted dispersive liquid-liquid microextraction combined with LC-MS/MS: application to forensic cases.

Analytical methods : advancing methods and applications·2026
Same journal

A lipid droplet-targeted fluorescent probe for selective imaging of cysteine fluctuations in drug-induced liver injury.

Analytical methods : advancing methods and applications·2026
Same journal

Study on the metal composition characteristics of high-end bags in Korean domestic distribution using portable XRF.

Analytical methods : advancing methods and applications·2026
Same journal

Spectrofluorimetric method for determination of the skin depigmenting substance - 4-hexylresorcinol - in cosmetic products.

Analytical methods : advancing methods and applications·2026
Same journal

Facile synthesis of diatomic FeSe-NC-900 nanozymes: peroxidase-mimicking activity and colorimetric assay of L-cysteine in real samples.

Analytical methods : advancing methods and applications·2026
See all related articles

Related Experiment Video

Updated: Jul 18, 2025

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

14.2K

Enhancing metabolite coverage in MALDI-MSI using laser post-ionisation (MALDI-2).

J C McKinnon1, H H Milioli2,3,4, C A Purcell2,3,4

  • 1Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Northfields Ave, Wollongong, NSW 2522, Australia. sellis@uow.edu.au.

Analytical Methods : Advancing Methods and Applications
|August 22, 2023
PubMed
Summary
This summary is machine-generated.

Matrix-assisted laser desorption/ionisation mass spectrometry imaging with laser post-ionisation (MALDI-2) significantly enhances spatial metabolomics. This advanced technique improves metabolite coverage and sensitivity for low molecular weight metabolites in complex tissues.

More Related Videos

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
09:08

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Published on: December 22, 2020

6.3K
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

9.5K

Related Experiment Videos

Last Updated: Jul 18, 2025

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

14.2K
Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
09:08

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Published on: December 22, 2020

6.3K
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

9.5K

Area of Science:

  • Metabolomics
  • Mass Spectrometry Imaging
  • Biochemistry

Background:

  • Matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) is crucial for understanding metabolic alterations in heterogeneous tissues.
  • Conventional MALDI-MSI has limitations in metabolite coverage and sensitivity, particularly for low molecular weight (LMW) metabolites.

Purpose of the Study:

  • To investigate the benefits of coupling laser post-ionisation (MALDI-2) with MALDI-MSI for spatial metabolomics.
  • To assess the improvements in metabolite coverage and detection sensitivity for LMW metabolites (< 600 m/z).

Main Methods:

  • Application of negative ion mode MALDI-2 coupled with MALDI-MSI to mouse kidney and liver tissues.
  • Comparison of MALDI-2 performance against conventional MALDI-MSI using specific matrices (e.g., N-(1-naphthyl) ethylenediamine dihydrochloride).
  • Analysis of datasets using the METASPACE platform and co-localisation analysis for tumour-specific metabolites.

Main Results:

  • MALDI-2 nearly doubled the number of on-tissue specific mass features in mouse kidney tissue compared to conventional MALDI.
  • MALDI-2 enhanced detection sensitivity and enabled imaging of LMW metabolites (e.g., uridine) previously undetectable.
  • MALDI-2 identified 34 unique metabolite m/z features in kidney tissue not consistently annotated in public datasets.
  • In liver tissue with metastatic breast cancer, MALDI-2 detected six tumour-specific metabolites and increased signal intensities up to 20-fold for others (e.g., glutamate).

Conclusions:

  • MALDI-2 offers significant improvements in sensitivity and metabolite coverage for spatial metabolomics.
  • This technique provides unprecedented insights into metabolic heterogeneity in complex biological samples.
  • MALDI-2 represents a powerful advancement for metabolite imaging in research and diagnostics.