Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Red cell vesiculation--a common membrane physiologic event.

G M Wagner, D T Chiu, M C Yee

    The Journal of Laboratory and Clinical Medicine
    |October 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Highly photostable wide-dynamic-range pH sensitive semiconducting polymer dots enabled by dendronizing the near-IR emitters.

    Chemical science·2017
    Same author

    What childhood obesity prevention programmes work? A systematic review and meta-analysis.

    Obesity reviews : an official journal of the International Association for the Study of Obesity·2015
    Same author

    Microfluidics for single-cell genetic analysis.

    Lab on a chip·2014
    Same author

    Variation in the heritability of body mass index based on diverse twin studies: a systematic review.

    Obesity reviews : an official journal of the International Association for the Study of Obesity·2013
    Same author

    Effects of aliasing on the fidelity of a two dimensional array of foci generated with a kinoform.

    Optics express·2011
    Same author

    Sibling donor cord blood banking for children with sickle cell disease.

    Pediatric pathology & molecular medicine·2003
    Same journal

    Studies on streptomycin; assay in body fluids.

    The Journal of laboratory and clinical medicine·2010
    Same journal

    Gastrocolic fistula; an experimental study.

    The Journal of laboratory and clinical medicine·2010
    Same journal

    An isolated heart perfusion system adapted to the determination of nongaseous metabolities; with sample data upon the isolated monkey heart.

    The Journal of laboratory and clinical medicine·2010
    Same journal

    A new type of glass cage for metabolism studies.

    The Journal of laboratory and clinical medicine·2010
    Same journal

    A simple electrocardiograph contact electrode for use with the smaller laboratory animal.

    The Journal of laboratory and clinical medicine·2010
    Same journal

    A primary standard for the colorimetric determination of hemoglobin.

    The Journal of laboratory and clinical medicine·2010
    See all related articles

    Red blood cell microvesicles, released during various conditions, contain specific proteins and may cause hypercoagulation. Spectrin cross-linking appears linked to this vesiculation process.

    Area of Science:

    • Hematology
    • Cell Biology
    • Biochemistry

    Background:

    • Red blood cell (RBC) membrane loss via microvesicles occurs in sickle cell disease, ATP-depleted cells, and during RBC storage.
    • Understanding the RBC vesiculation process is crucial for comprehending various hemolytic disorders.

    Purpose of the Study:

    • To investigate the mechanism and consequences of red blood cell membrane vesiculation.
    • To characterize the protein and phospholipid composition of generated vesicles.
    • To assess the pathophysiological impact of these vesicles.

    Main Methods:

    • Vesicles were isolated from pathologic and normal RBCs subjected to various stressors (e.g., ATP depletion, heat, pH changes, diamide).
    • Membrane protein composition was analyzed using SDS-PAGE and immunoblotting.

    Related Experiment Videos

  • Spectrin cross-linking was assessed via thiol disulfide exchange chromatography.
  • Phospholipid analysis was performed using thin-layer chromatography.
  • Procoagulant activity was measured using a Russell's viper venom assay.
  • Cell rigidity was evaluated using ektacytometry.

    • Main Results:

    • Vesicles consistently contained band 3, glycophorin A, and band 4.1, with depleted spectrin.
    • Oxidative cross-linking of spectrin was observed in vesiculated RBCs, suggesting a role in the mechanism.
    • Phospholipid composition of sickle cell and acid-induced vesicles resembled normal RBCs.
    • All examined vesicles significantly shortened Russell's viper venom clotting time, indicating procoagulant activity.
    • Acid-induced vesiculation resulted in increased rigidity of remaining RBCs.

    Conclusions:

    • RBC vesiculation involves spectrin cross-linking and results in procoagulant vesicles.
    • These vesicles may contribute to hypercoagulation observed in hemolytic disorders.
    • The vesiculation process can increase the rigidity of remaining RBCs, potentially leading to their clearance from circulation.