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Updated: Jul 18, 2025

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
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Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

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CIRC_0085323 SILENCING INHIBITS TNF-Α-INDUCED NORMAL HUMAN COLONIC EPITHELIAL CELL INFLAMMATION AND APOPTOSIS THROUGH

Qi An1, Shengsen Yang1, Jianan Tao1

  • 1Department of Digestive System, Qinghai University Affiliated Hospital, Xining City, China.

Shock (Augusta, Ga.)
|August 22, 2023
PubMed

Insights

Circular RNA circ_0085323 promotes ulcerative colitis (UC) by sponging microRNA-495-3p and regulating the TRAF3 axis. Silencing circ_0085323 alleviates inflammation and cell damage in UC models.

Area of Science:

  • Molecular Biology
  • Gastroenterology
  • Cell Biology

Background:

  • Circular RNAs (circRNAs) are implicated in ulcerative colitis (UC) pathogenesis.
  • The specific role of circ_0085323 in UC remains unelucidated.

Purpose of the Study:

  • To investigate the role and mechanism of circ_0085323 in UC development.
  • To explore the regulatory axis involving circ_0085323, microRNA-495-3p (miR-495-3p), and TNF receptor-associated factor 3 (TRAF3) in UC-like conditions.

Main Methods:

  • Utilized normal human colonic epithelial cells (NCM460) stimulated with TNF-α to mimic UC in vitro.
  • Quantified gene and protein expression using qRT-PCR and Western blotting.
  • Assessed cell viability, proliferation, apoptosis, and inflammatory cytokine release (IL-1β, IL-6, IL-8).
  • Investigated molecular interactions using dual-luciferase reporter and RNA immunoprecipitation assays.

Main Results:

  • Circ_0085323 was upregulated in UC patient tissues and TNF-α-treated NCM460 cells.
  • Knockdown of circ_0085323 reduced TNF-α-induced NCM460 cell injury, apoptosis, and inflammation.
  • Circ_0085323 functioned as a miR-495-3p sponge, and miR-495-3p targeted TRAF3.
  • The circ_0085323/miR-495-3p/TRAF3 axis mediated cellular responses to TNF-α stimulation.

Conclusions:

  • Circ_0085323 knockdown mitigates TNF-α-induced colonic epithelial cell injury via the miR-495-3p/TRAF3 pathway.
  • Circ_0085323 represents a potential therapeutic target for ulcerative colitis.

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