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Related Concept Videos

Bone Remodeling01:40

Bone Remodeling

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Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
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Related Experiment Video

Updated: Jul 18, 2025

Author Spotlight: PEGASOS Tissue Clearing Technique to Visualize Bone Remodeling
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A 3-D Visualization Technique for Bone Remodeling in a Suture Expansion Mouse Model.

Ziduan Ding1, Ruomei Li1, Yufeng Duan1

  • 1Department of Orthodontics, China Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University.

Journal of Visualized Experiments : Jove
|August 22, 2023
PubMed
Summary

This study introduces a novel mouse model and 3D imaging technique to visualize bone remodeling in craniofacial sutures during expansion. It reveals key cellular changes, enhancing our understanding of orthopedic treatments.

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Area of Science:

  • Craniofacial Development and Orthopedics
  • Tissue Engineering and Regenerative Medicine
  • Biomechanical Engineering

Background:

  • Craniofacial sutures are vital growth centers for skull and facial bones, housing stem cells crucial for intramembranous ossification.
  • These sutures are key targets for orthopedic interventions like rapid maxillary expansion, yet the underlying bone remodeling dynamics are poorly understood.
  • Current histological methods are limited in capturing comprehensive, three-dimensional changes within sutures during expansion.

Purpose of the Study:

  • To establish a standardized mouse model for sagittal suture expansion.
  • To develop and apply a 3D visualization technique for analyzing bone remodeling post-expansion.
  • To investigate the mechanobiological responses of suture stem cells and osteoprogenitors to tensile forces.

Main Methods:

  • Development of a standardized mouse model for sagittal suture expansion.
  • Application of the PEGASOS tissue clearing method for whole-mount visualization.
  • Combined whole-mount EdU staining and calcium chelating double labeling to track cell proliferation and bone formation.

Main Results:

  • Successful establishment of a reproducible mouse model for studying suture expansion.
  • Comprehensive 3D visualization of highly proliferating cells and new bone formation across calvarial bones post-expansion.
  • Detailed mapping of cellular activities and bone deposition in response to mechanical loading.

Conclusions:

  • The developed protocol provides a robust platform for studying suture biology and mechanobiology.
  • This approach offers unprecedented insights into the dynamic processes of bone remodeling in craniofacial sutures.
  • The findings have significant implications for optimizing orthopedic therapies targeting craniofacial growth and development.