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Updated: Jul 18, 2025

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
Published on: March 14, 2019
Mutations in microRNA-128-2-3p identified with amplification-free hybridization assay
Sofie Slott1, Cecilie Schiøth Krüger-Jensen1, Izabela Ferreira da Silva2,3,4
1Department of Chemistry, Technical University of Denmark, Kgs Lyngby, Denmark.
Abstract:
We describe a quantitative detection method for mutated microRNA in human plasma samples. Specific oligonucleotides designed from a Peyrard-Bishop model allowed accurate prediction of target:probe recognition affinity and specificity. Our amplification-free tandem bead-based hybridization assay had limit of detection of 2.2 pM. Thereby, the assay allowed identification of single-nucleotide polymorphism mismatch profiles in clinically relevant microRNA-128-2-3p, showing terminal mutations that correlate positively with inflammatory colitis and colorectal cancer.

