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Updated: Jul 18, 2025
Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation
Published on: April 5, 2024
Nd:YAG-photobiomodulation enhanced ADSCs multilineage differentiation and immunomodulation potentials
Linhai He1, Yi Zheng2, Meng Liu3
1First Clinical Division, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, People's Republic of China.
Nd:YAG photobiomodulation enhances adipose tissue-derived stem cells (ADSCs) for therapeutic use. This study shows it improves stem cell differentiation and anti-inflammatory properties, potentially aiding clinical applications.
Area of Science:
- Regenerative Medicine
- Biophotonics
- Stem Cell Biology
Background:
- Adipose tissue-derived stem cells (ADSCs) hold promise for regenerative therapies.
- Photobiomodulation (PBM) is an emerging therapeutic modality.
- Optimizing ADSC potential for clinical applications requires further investigation.
Purpose of the Study:
- To investigate the effects of Nd:YAG (1064 nm) photobiomodulation on ADSC multilineage differentiation.
- To assess the impact of Nd:YAG PBM on ADSC immunomodulation potentials.
- To evaluate the therapeutic efficacy of PBM-treated ADSCs in a colitis model.
Main Methods:
- In vitro experiments exposed ADSCs to varying Nd:YAG PBM doses (0.5-4 J/cm²).
- Assessed ADSC proliferation, migration, osteogenic, and adipogenic differentiation using qPCR and staining.
- Evaluated immunomodulation via cytokine arrays and tested in a DSS-induced colitis mouse model.
Main Results:
- Nd:YAG PBM dose-dependently promoted ADSC proliferation and migration, with 1 J/cm² being optimal.
- PBM enhanced ADSC osteogenic and brown adipose adipogenic differentiation.
- PBM-treated ADSCs demonstrated improved anti-inflammatory capacity and alleviated colitis severity in vivo.
Conclusions:
- Nd:YAG photobiomodulation shows potential to enhance ADSC multilineage differentiation and immunomodulation.
- These findings suggest PBM could improve ADSC therapeutic efficacy for clinical applications.
- Further research is needed to elucidate the underlying mechanisms of PBM-induced ADSC enhancement.
