A colorimetric analytical method based on a TCPP-CuCo₂O₄-like peroxidase for the detection of trichlorfon

Area of Science:

• Analytical Chemistry
• Environmental Science
• Materials Science

Background:

• Trichlorfon is a widely used organophosphate pesticide with potential environmental and health risks.
• Accurate detection methods for trichlorfon are crucial for food safety and environmental monitoring.
• Existing detection methods may lack sensitivity, specificity, or practicality for field applications.

Purpose of the Study:

• To develop a highly sensitive colorimetric sensing platform for trichlorfon detection.
• To utilize a novel functionalized nanomaterial for enhanced sensing capabilities.
• To establish a reliable method for trichlorfon analysis in complex matrices like vegetables.

Main Methods:

• Synthesis of 5,10,15,20-Tetracarboxyphenylporphyrin (TCPP) functionalized CuCo2O4 (TCPP-CuCo2O4) nanomaterial.
• Design of a colorimetric assay based on the inhibition of thiocholine (TCh)-induced redox reaction catalyzed by acetylcholinesterase (AChE).
• Spectrophotometric monitoring of the color change associated with the redox reaction at 652 nm.

Main Results:

• The TCPP-CuCo2O4 based sensor demonstrated high sensitivity for trichlorfon detection.
• A wide linear detection range of 40-4000 ng mL−1 was achieved with a high correlation coefficient (0.9904).
• The method showed good applicability for detecting trichlorfon in real vegetable samples, indicating practical potential.

Conclusions:

• The developed colorimetric sensing platform offers a sensitive, efficient, and cost-effective method for trichlorfon detection.
• The inhibition of AChE activity by trichlorfon provides a robust signal for quantitative analysis.
• This approach holds promise for routine monitoring of trichlorfon residues in agricultural products and environmental samples.