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Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

Nanogels designed for cell-free nucleic acid sequestration.

Yuhang Huang¹, Shangyu Li², Logan W C Zettle²

¹Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario M5S 3E5, Canada. eugenia.kumacheva@utoronto.ca.

|August 23, 2023

Summary

New cationic nanogels effectively scavenge cell-free deoxyribonucleic acid (cfDNA) from chronic wounds. Their unique structure sequesters cfDNA internally, offering a promising approach for improved wound healing treatments.

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Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

Area of Science:

Biomaterials Science
Wound Healing Research
Nanotechnology

Background:

Chronic wounds show elevated cell-free deoxyribonucleic acid (cfDNA) levels, contributing to persistent inflammation and delayed healing.
Current nanoscopic particles for cfDNA scavenging are limited by surface adsorption, hindering efficient uptake.
Nanogels offer potential for enhanced cfDNA binding within their internal structure, an area yet to be explored for wound treatment.

Purpose of the Study:

To develop and evaluate novel cationic nanogels as efficient cfDNA scavengers for chronic wound treatment.
To investigate the cfDNA sequestration capabilities of nanogels, considering their internal network structure.
To assess the biocompatibility and potential application of these nanogels in wound dressings.

Main Methods:

Synthesized cationic nanogels from a copolymer of chitosan and poly{2-[(acryloyloxy)ethyl]trimethylammonium chloride}.
Characterized nanogel properties, including charge retention at physiological wound conditions.
Evaluated cfDNA scavenging efficiency, focusing on internal sequestration within the nanogel network.

Main Results:

The cationic nanogels maintained positive charge in simulated chronic wound environments, facilitating electrostatic cfDNA binding.
Nanogel network structure enabled cfDNA sequestration within the nanogel interior, surpassing surface-only adsorption.
Enhanced cfDNA scavenging efficiency was observed, influenced by the nanogel pore size relative to cfDNA dimensions.

Conclusions:

Cationic nanogels demonstrate superior cfDNA scavenging capabilities due to internal sequestration.
The nanogels exhibit biocompatibility, making them suitable for chronic wound management.
These nanogels represent a promising component for advanced wound dressing technologies to promote healing.