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Host Transcriptome Analysis of Ferret Tissues Following Henipavirus Infection.

Tian S Zeng1, D S Yang1, A A Kelvin1

  • 1Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|August 23, 2023
PubMed
Summary

Ferrets serve as valuable preclinical models for studying viral infections like Hendra and Nipah viruses. Genome-wide gene expression analysis reveals early interferon pathway activation and later inflammation in ferret lung tissues.

Keywords:
HenipavirusHost ImmunityInterferonRNA analysisTranscriptome

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Area of Science:

  • Virology
  • Genomics
  • Immunology

Background:

  • Ferrets are susceptible to human respiratory viruses, making them ideal experimental models.
  • Advancements in ferret genomics facilitate whole-genome gene expression analysis.
  • Limited ferret-specific reagents previously hindered comprehensive studies.

Purpose of the Study:

  • To describe methods for genome-wide gene expression analysis in ferrets infected with Hendra virus (HeV) and Nipah virus (NiV).
  • To analyze host gene expression profiles in lung and brain tissues.
  • To characterize the host response to HeV and NiV infection in a preclinical model.

Main Methods:

  • Experimental infection of ferrets with HeV and NiV.
  • Whole-genome gene expression analysis using RNA sequencing (RNAseq) on lung and brain tissues.
  • Bioinformatic analysis of RNAseq data to identify host gene expression profiles.

Main Results:

  • Detailed RNAseq methods are provided.
  • Representative host gene expression profiles from lung tissues are presented.
  • Early activation of interferon pathways and subsequent inflammation-related pathways were observed in lung tissues.

Conclusions:

  • Ferrets are a suitable preclinical model for studying HeV and NiV infections.
  • RNAseq analysis provides insights into the host response dynamics.
  • Findings highlight the utility of ferrets for investigating viral pathogenesis and host-immune interactions.