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Updated: Jul 18, 2025
Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse
Published on: August 15, 2018
Histological study on the reginal difference in the localization of mucosal enteric glial cells and their sheath
Youhei Mantani1, Nobuhiko Ohno2,3, Tomohiro Haruta4
1Laboratory of Histophysiology, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, Hyogo, Japan.
Abstract:
The present study aimed to histologically clarify the regional specificity of the mucosal enteric glial cells (mEGCs) in the rat intestine with serial block-face scanning electron microscopy (SBF-SEM). SBF-SEM analysis with the ileum, the cecum and the descending colon revealed that mEGC nuclei were more abundant in the data stacks from the apical portion of the villus and the lateral portion of the crypt of the ileum. mEGCs exhibited a high rate of coverage over the nerve bundle around the lateral portion of the ileal crypt, but showed an extremely low level of coverage in the luminal portion of the cecum. These findings evidenced regional differences in the localization of mEGCs and in their sheath structure in the rat intestine.
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