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Updated: Jul 18, 2025
Software-Assisted Quantitative Measurement of Osteoarthritic Subchondral Bone Thickness
Published on: March 18, 2022
Identification of basement membrane-related biomarkers associated with the diagnosis of osteoarthritis based on
Xiaojing Huang1, Hongming Meng1,2, Zeyu Shou2
1Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou City, 325000, Zhejiang Province, China.
Background:
Osteoarthritis is a very common clinical disease in middle-aged and elderly individuals, and with the advent of ageing, the incidence of this disease is gradually increasing. There are few studies on the role of basement membrane (BM)-related genes in OA.
Method:
We used bioinformatics and machine learning methods to identify important genes related to BMs in OA patients and performed immune infiltration analysis, lncRNA‒miRNA-mRNA network prediction, ROC analysis, and qRT‒PCR.
Result:
Based on the results of machine learning, we determined that LAMA2 and NID2 were the key diagnostic genes of OA, which were confirmed by ROC and qRT‒PCR analyses. Immune analysis showed that LAMA2 and NID2 were closely related to resting memory CD4 T cells, mast cells and plasma cells. Two lncRNAs, XIST and TTTY15, were simultaneously identified, and lncRNA‒miRNA‒mRNA network prediction was performed.
Conclusion:
LAMA2 and NID2 are important potential targets for the diagnosis and treatment of OA.
