Synthesis and secretion of sulphated glycoproteins by rabbit oviduct explants in vitro

The ability of rabbit oviduct explants to incorporate radiolabelled precursors into specific secretory products was investigated. Ampullary and isthmic oviduct segments were cultured in the presence of [3H]glucosamine or [35S]sodium sulphate. Medium samples were analysed for the presence of secreted, labelled macromolecules. Explants incorporated the [3H]glucosamine and secreted labelled glycoproteins in vitro. SDS gel electrophoresis and subsequent fluorographic analysis of culture medium demonstrated a differential secretion of glycoproteins between the ampulla and the isthmus. Although ampullary tissue secreted a greater amount of labelled glycoproteins during the sampling period, the major secretory constituent of Mr approximately 66,000 was common to both oviduct segments. Tissue incubated with [35S]sodium sulphate also secreted a labelled glycoprotein or subunit of Mr approximately 66,000. The results indicate that rabbit oviduct explants are capable of synthesis and secretion of specific sulphated glycoproteins in vitro and that there is a difference in the type and amount of secretion produced between the two oviduct segments.