Trails to Research: an Inquiry-Based Course Using Zebrafish To Provide Research Experience to Tribal College Students

Embryonic development is fascinating to follow and highly engaging and, therefore, lends itself for undergraduate students' first steps in experimental science. We developed the "Trails to Research" inquiry-based course, which exposes students to life science research using zebrafish as model organism. Zebrafish are ideal in the classroom: they are easy to maintain, their embryos develop rapidly, and they are easily manipulated. Further, they lend themselves to teach about embryo development and experimental design. We developed the course for undergraduates at 2-year colleges and, therefore, for students with little or no research experience. In this 5-day intensive course (which is taught during summers as a stand-alone course), students design treatment experiments for zebrafish embryos with known teratogens and with substances they select. The course comprises three modules that overlap over the 5 days: (i) introduction to developmental biology, model organisms, toxicology, and experimental design, (ii) zebrafish embryo experimental setup, and (iii) collecting, analyzing, and presenting data. Student learning was significant in the areas of experimental design, working with model systems, working with zebrafish embryos, using laboratory equipment, and presenting the results of their experiments using effective methods.