Hybrid Inquiry-Based Laboratory Curriculum Highlights Scientific Method Using Bacterial Conjugation as a Model

Area of Science:

• Microbiology Education
• Inquiry-Based Learning

Background:

• Traditional microbiology labs often prioritize technical skills over scientific thinking.
• Integrating scientific method into laboratory curricula is crucial for developing student competencies.

Purpose of the Study:

• To design and assess a six-session inquiry-based laboratory (IBL) curriculum for upper-level microbiology.
• To fully immerse students in the scientific process using bacterial conjugation as a model system.
• To evaluate student learning in scientific method, experimental design, data analysis, bacterial conjugation, and scientific communication.

Main Methods:

• Developed a six-session IBL curriculum combining online discussions and in-person lab sessions.
• Utilized bacterial conjugation as the model system for scientific inquiry.
• Assessed student learning via pre- and post-IBL quizzes and the Laboratory Course Assessment Survey.

Main Results:

• The IBL curriculum led to positive student learning gains across key objectives.
• Students showed improved understanding of the scientific method and experimental design.
• The curriculum effectively integrated scientific thinking with technical skills in microbiology.

Conclusions:

• The designed IBL curriculum is effective in enhancing microbiology students' scientific thinking and learning.
• This approach successfully integrates the scientific method into laboratory coursework.
• The model system of bacterial conjugation proved suitable for inquiry-based learning in microbiology.