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Updated: Jul 18, 2025

Comparing the Affinity of GTPase-binding Proteins using Competition Assays
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Allosteric site variants affect GTP hydrolysis on Ras

Christian W Johnson1, Susan K Fetics2, Kathleen P Davis2

  • 1Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA.

Protein Science : a Publication of the Protein Society
|August 24, 2023
PubMed
Summary

Manipulating the allosteric site of HRAS GTPase impacts GTP hydrolysis. Allosteric site closure increases HRAS GTP hydrolysis, while RAF kinase binding inhibits it, potentially stabilizing the GTP-bound state in cancer.

Keywords:
HRASKRASRAFallosteric siteallosteryhydrolysismutationsmall GTPase

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