Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Acute Kidney Injury IV: Diagnostic Studies and Prevention01:30

Acute Kidney Injury IV: Diagnostic Studies and Prevention

34
Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
34
Acute Kidney Injury II: Pathophysiology01:29

Acute Kidney Injury II: Pathophysiology

28
Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
28
Chronic Kidney Disease I: Introduction01:25

Chronic Kidney Disease I: Introduction

35
Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage kidney disease (ESKD). At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate...
35
Chronic Kidney Disease III: Interprofessional Care01:28

Chronic Kidney Disease III: Interprofessional Care

42
Chronic kidney disease (CKD) requires collaborative and comprehensive management. CKD progresses through stages and can lead to end-stage kidney disease (ESKD) if untreated. Interprofessional collaboration and patient education are crucial, enabling patients to manage their health and improve their quality of life.Diagnostic approach for chronic kidney diseaseThe diagnosis of CKD primarily focuses on the glomerular filtration rate (GFR), which assesses kidney function by measuring how well...
42
Acute Kidney Injury I: Introduction01:22

Acute Kidney Injury I: Introduction

28
Introduction:Acute Kidney Injury (AKI) describes a swift decrease in kidney function occurring over hours to days, characterized by the kidneys' failure to remove waste products from the bloodstream. This leads to dangerous complications like metabolic acidosis, fluid overload, and electrolyte imbalances, such as hyperkalemia, which can cause life-threatening arrhythmias. AKI is common in both hospital and outpatient settings, often triggered by dehydration, sepsis, or exposure to nephrotoxic...
28
External Anatomy of the Kidney01:21

External Anatomy of the Kidney

1.2K
The kidneys are a pair of bean-shaped organs in the human body that play a critical role in maintaining overall health. They filter out waste products from the blood, regulate blood pressure, maintain electrolyte balance, and stimulate the production of red blood cells.
The kidneys are located in the retroperitoneal space on either side of the vertebral column, protected posteriorly by the 11th and 12th ribs. The right kidney sits slightly lower than the left owing to the presence of the liver...
1.2K
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Endogenous Adenine Mediates Kidney Injury In Diabetic Models And Predicts Diabetic Kidney Disease In Patients

Endogenous adenine mediates kidney injury in diabetic models and predicts diabetic kidney disease in patients

Kumar Sharma1,2, Guanshi Zhang1,2, Jens Hansen3

  • 1Center for Precision Medicine and.

The Journal of Clinical Investigation
|August 24, 2023

Related Experiment Videos

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

212
Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease
08:15

Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease

Published on: May 10, 2024

611
Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

17.7K

View abstract on PubMed

Summary
This summary is machine-generated.

Urine adenine/creatinine ratio (UAdCR) may predict end-stage kidney disease (ESKD) in diabetic kidney disease (DKD) patients. Lowering UAdCR with empagliflozin and inhibiting adenine production reduced kidney injury in mice, suggesting adenine

Keywords:
Chronic kidney diseaseDiabetesFibrosisNephrology

Related Experiment Videos

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

212
Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease
08:15

Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease

Published on: May 10, 2024

611
Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

17.7K

Area of Science:

  • Nephrology
  • Metabolomics
  • Molecular Biology

Background:

  • Diabetic kidney disease (DKD) is a leading cause of end-stage kidney disease (ESKD) and mortality.
  • Current biomarkers for high-risk DKD patients, particularly those without macroalbuminuria, are limited.
  • Understanding mechanistic biomarkers is crucial for early intervention in DKD.

Purpose of the Study:

  • To investigate urine adenine/creatinine ratio (UAdCR) as a mechanistic biomarker for ESKD in diabetic patients.
  • To explore the role of adenine in DKD pathogenesis.
  • To assess the effect of empagliflozin on UAdCR in nonmacroalbuminuric individuals.

Main Methods:

  • Analysis of UAdCR in large diabetic cohorts (CRIC, SMART2D, American Indian Study).
  • Spatial metabolomics and single-cell transcriptomics to localize adenine and identify related pathways.
  • In vivo studies using diabetic mice treated with adenine production inhibitors.

Main Results:

  • Highest UAdCR tertiles were associated with increased risk of ESKD and mortality in CRIC and SMART2D cohorts.
  • This association persisted in patients without macroalbuminuria across all three studies.
  • Empagliflozin reduced UAdCR in nonmacroalbuminuric participants; adenine was localized to kidney pathology and implicated mTOR pathway.

Conclusions:

  • Urine adenine/creatinine ratio is a potential mechanistic biomarker for predicting ESKD in diabetic kidney disease.
  • Endogenous adenine may play a causative role in DKD progression, potentially via the mTOR pathway.
  • Targeting adenine production could be a novel therapeutic strategy for diabetic kidney disease.