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A dual-targeting nanobiosensor for Gender Determination applying Signal Amplification Methods and integrating
Fatima Bazzi1, Morteza Hosseini2, Bahman Ebrahimi-Hoseinzadeh3
1Nanobiosensors Lab, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, P.O. Box 14399-56191, Tehran, Iran.
Mikrochimica Acta
|August 24, 2023
Summary
A novel dual-targeting nanobiosensor simultaneously detects AMELX and AMELY genes using gold and silver nanoclusters. This innovative biosensor offers high sensitivity and specificity for gene detection.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Molecular Diagnostics
Background:
- Simultaneous detection of multiple biomarkers is crucial for accurate disease diagnosis.
- Existing methods for gene detection can be complex, time-consuming, and require expensive equipment.
- Development of sensitive, specific, and cost-effective biosensors is an ongoing challenge.
Purpose of the Study:
- To develop a dual-targeting nanobiosensor for the simultaneous detection of AMELX and AMELY genes.
- To utilize gold nanoclusters (AuNCs) and silver nanoclusters (AgNCs) for distinct fluorescence signaling.
- To employ catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) for signal amplification.
Main Methods:
- Designed a nanobiosensor integrating CHA and HCR for enzyme-free signal amplification.
- Utilized AuNCs and AgNCs for distinct fluorescence detection of AMELX and AMELY.
- Leveraged aggregation-induced emission (AIE) for enhanced fluorescence signals upon target binding.
- Validated the assay's performance using real biological samples.
Main Results:
- Achieved simultaneous detection of AMELX and AMELY genes with distinct fluorescence signals.
- Demonstrated high sensitivity with detection limits as low as 3.16 fM for AMELX and 23.6 fM for AMELY.
- The nanobiosensor exhibited specificity, stability, and rapid results.
- Showcased excellent performance in real sample analysis.
Conclusions:
- The developed dual-targeting nanobiosensor provides a simple, inexpensive, and effective platform for simultaneous gene detection.
- The assay's high sensitivity and specificity make it promising for clinical diagnostics.
- This versatile design holds potential for detecting other biomarkers using nanobiosensor technology.
