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Related Experiment Video

Updated: Jul 18, 2025

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

SARS-CoV-2 ORF8 accessory protein is a virulence factor.

M Bello-Perez¹, J Hurtado-Tamayo¹, A Z Mykytyn²

¹Department of Molecular and Cell Biology, National Center for Biotechnology (CNB-CSIC), Campus Universidad Autónoma de Madrid , Madrid, Spain.

|August 25, 2023

Summary

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ORF8 protein contributes to COVID-19 virulence by promoting inflammation and activating macrophages. Targeting ORF8 may offer new therapeutic strategies for COVID-19.

Keywords:

SARS-CoV-2 accessory proteins coronavirus viral pathogenesis virulence

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A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

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Published on: January 9, 2019

Area of Science:

Virology
Immunology
Pathogenesis

Background:

The role of SARS-CoV-2 ORF8 in COVID-19 pathogenesis remains unclear.
Natural SARS-CoV-2 isolates with ORF8 deletions are linked to milder disease, suggesting ORF8 contributes to virulence.

Purpose of the Study:

To investigate the role of SARS-CoV-2 ORF8 in inflammation and macrophage activation.
To determine if ORF8 is a potential therapeutic target for COVID-19.

Main Methods:

Utilized K18-hACE2 transgenic mice model.
Employed organoid-derived human airway cell cultures.

Main Results:

Demonstrated ORF8 protein involvement in promoting inflammation.
Showed ORF8 activates macrophages in experimental systems.

Conclusions:

SARS-CoV-2 ORF8 plays a significant role in COVID-19 pathogenesis through inflammation and macrophage activation.
ORF8 protein is identified as a potential therapeutic target for COVID-19 treatment.