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Related Experiment Video

Updated: Jul 18, 2025

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Published on: September 28, 2021

A Rapid Method for Measuring In Vitro Growth in Entomopathogenic Fungi.

Anna R Slowik^1,2,3, Helen Hesketh², Steven M Sait³

¹Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg, Denmark.

|August 25, 2023

Summary

This study introduces a rapid spectrophotometry method for measuring entomopathogenic fungal growth in microplates. This high-throughput technique accurately quantifies fungal biomass, aiding virulence studies.

Keywords:

Metarhizium bioassay technique biomass quantification filamentous fungi fungal growth microplate reader microspectrophotometry spectrophotometry

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Comparison of Methods for Isolating Entomopathogenic Fungi from Soil Samples

Comparison of Methods for Isolating Entomopathogenic Fungi from Soil Samples

Published on: January 6, 2022

Area of Science:

Agricultural Science
Mycology
Biotechnology

Background:

Quantifying entomopathogenic fungi growth is vital for understanding virulence.
Traditional methods are time-consuming and limited in scope.

Purpose of the Study:

Introduce a high-throughput spectrophotometry method for rapid fungal growth measurement.
Validate spectrophotometric measurements against dry weight biomass.
Investigate species- and isolate-specific growth patterns in Metarhizium.

Main Methods:

Utilized 96-well microplates for small-volume liquid cultures.
Measured fungal growth via optical density (OD).
Correlated OD with dry weight biomass to generate standard curves.

Main Results:

Spectrophotometry accurately predicted fungal biomass.
Distinct growth patterns were observed among six Metarhizium isolates.
The logistic growth phase was accurately assessed within 80 hours.

Conclusions:

Spectrophotometry provides an effective, reproducible, and precise method for rapid filamentous fungal growth measurement.
This technique is a valuable tool for studying entomopathogenic fungal growth dynamics.
The method aids in investigating factors influencing fungal growth and virulence.