A genetically encodable and fluorogenic photo-crosslinker via photo-induced defluorination acyl fluoride exchange
Jielin Fu1, Sitong Li1, Lijun Deng1
1Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, P. R. China. zhipengy@scu.edu.cn.
Abstract:
We report a genetically encodable m-trifluoromethylaniline modified L-lysine (m-TFMAK) which defluorinates upon light activation and covalently conjugates to native residues via acyl fluoride exchange. The encoded m-TFMAK photo-crosslinks with temporal controllability, residue selectivity, and fluorogenic tracking features, making it suitable for identifying protein interactions in living systems.


