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Updated: Jul 18, 2025

Author Spotlight: Investigating the Effects of Compounds on Intestinal Tissue Using 3D Human Cell Line Models
Published on: September 1, 2023
Ex vivo gliadin stimulation of intestinal cells
1Department of Chemistry, Institute for Biophysical Dynamics, University of Chicago, Chicago, IL, United States; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, United States; Howard Hughes Medical Institute, University of Chicago, Chicago, IL, United States.
Abstract:
Celiac disease is an autoimmune response to gluten proteins. While causes for celiac disease have been identified, there is no effective treatment other than diet control. In vitro models for celiac disease are important for quickly gaining understanding of the disease mechanism and testing potential treatments. Here we describe an ex vivo stimulation of intestinal epithelial cells with gliadin peptides as a method to induce celiac disease features in vitro.

