Modulation of TLR4/NFκB Pathways in Autoimmune Myocarditis

Area of Science:

• Cardiology
• Immunology
• Pharmacology

Background:

• Myocarditis is an inflammatory and oxidative disorder causing immune cell infiltration and organ dysfunction.
• Current treatments for myocarditis often have limitations, necessitating exploration of novel therapeutic agents.

Purpose of the Study:

• To investigate the therapeutic potential of Coriolus versicolor in an experimental model of autoimmune myocarditis (EAM).
• To elucidate the molecular mechanisms underlying the anti-inflammatory and cardioprotective effects of Coriolus versicolor.

Main Methods:

• Experimental autoimmune myocarditis was induced in animals using pig cardiac myosin.
• Coriolus versicolor (200 mg/Kg) was administered orally for 21 days.
• Molecular pathways, cytokine levels, oxidative stress markers, antioxidant enzyme activities, and histological changes were assessed.

Main Results:

• Coriolus versicolor administration reduced TLR4/NF-κB pathway activation and pro-inflammatory cytokines (INF-γ, TNF-α, IL-6, IL-17, IL-2), while restoring anti-inflammatory IL-10.
• Treatment decreased lipid peroxidation and nitrite levels, enhanced antioxidant enzyme activities (SOD, CAT), and increased GSH levels.
• Histological injury, immune cell infiltration (CD4+, CD68+), and apoptosis were significantly reduced.

Conclusions:

• Coriolus versicolor exhibits significant anti-inflammatory, antioxidant, and anti-apoptotic effects in autoimmune myocarditis.
• The compound modulates the TLR4/NF-κB signaling pathway, highlighting its therapeutic potential for myocarditis.